THE GROWTH OF PIMPINELLA ALPINA HOST CALLUS AT VARIOUS TREATMENTS OF PLANT GROWTH REGULATOR CONCENTRATIONS OF NAA. 2,4 D AND ITS COMBINATION WITH BAP

Purwaceng (Pimpinella alpina) is an herbal plant that has aphrodisiac, diuretic, and tonic properties. The imbalance between the growth exploitation of this makes it included in Appendix I (endangered), so its conservation will be crucial. One method tissue culture. This considered one way to induct callus. research objective find effect various treatments regulator concentrations NAA. 2,4 D combination with BAP on purwoceng Concentration NAA (0.5 mg L-1, 1 2 L-1), 2,4-D (0.4 0.8 1.2 L-1 ), a NAA: BAP, 3 BAP) 2, 4- (1 LL : 0.5 L into Murashige Skoog medium. Then purwaceng leaf explants were planted medium until was seen. shows concentration different influence callus recognized by day induction, colour texture. MS media can grow faster (17 days) friable